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https://repository.unad.edu.co/handle/10596/29806| Title: | Dose Answer of Different Inhibitors for Metabolic Studies in Astrocyte With L-Lactato in Perinatal Concentrations. Dosis Respuesta de Diferentes Inhibidores para Estudios Metabólicos en Astrocitos con L-Lactato en Concentraciones Perinatales. |
| metadata.dc.creator: | Tovar Franco MSC PhD, Jairo Alfonso |
| Keywords: | astrocyte; inhibitor; lipogenic; metabolism; respiration.;astrocito; inhibidor; lipogénesis; metabolismo; respiración. |
| Publisher: | Universidad Colegio Mayor de Cundinamarca |
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| metadata.dc.format.*: | application/pdf |
| metadata.dc.type: | info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion article article article |
| Description: | The transport systems such as the mitochondrial/cytosolic shuttles are important for the integrating functions of the brain in development. To facilitate their study and to know the rol that these transport systems could have in cerebral cells, radioactive tracers in primary cultured have been used. In this work the oxidation (respiration) and lipogenic rates were evaluated using lactate which is the main cerebral substrate during the presucking in astrocytes in primary cultured, being focused in determinating the minimal inhibitors concentrations that interact with the main shuttles without toxic effects on tested cells; these concentrations must allow us to observe metabolic effects at the same time. Uses of L-[U-14C]-lactato (1 MCi), L-lactato (10.5 mM) in absence and presence of different concentrations of enzymatic inhibitors as dichloroacetate (DCA), an inhibitor of pyruvate dehydrogenase kinase and aminooxyacetate (AOA), an inhibitor of aspartate aminotransferase and inhibitors of the mitochondrial transport like butylmalonate (BM), an inhibitor of the dicarboxilic acid transport and specifically of L-malate and the 1,2,3-benzene tricarboxylate (BT) an inhibitor of the tricarboxilic acid transport. The results suggest that concentrations of 1 mM of DCA and of 5 mM of AOA, BM and BT are enough to evaluate the metabolic effects in cerebral cells during the perinatal period. Los sistemas de transporte como las lanzaderas mitocondrial/citosólicas son importantes para las funciones integrativas del cerebro en desarrollo. Para facilitar su estudio y conocer el papel que estos sistemas de transporte puedan tener en células cerebrales se ha empleado trasadores radiactivos en cultivos primarios. En este trabajo se evaluaron las velocidades de oxidación (respiración) y lipogénesis utilizando lactato que es el principal sustrato cerebral durante la prelactancia en astrocitos en cultivo primario. Se determinó las concentraciones mínimas de inhibidores que interactúan con las principales lanzaderas de manera que no fueran tóxicos para las células, pero que su vez permitieran observar efectos metabólicos. Se utilizó L-[U-14C]- lactato (1 MCi), L-lactato (10.5 mM) en ausencia y presencia de diferentes concentraciones de inhibidores enzimáticos como el dicloroacetato (DCA), un inhibidor de la piruvato deshidrogenasa quinasa y el aminooxiacetato (AOA), un inhibidor de la aspartato aminotransferasa e inhibidores del transporte mitocondrial como el butilmalonato (BM), un inhibidor del transporte de dicarboxilatos y específicamente del L-malato y el 1,2,3-benceno tricarboxilato (BT) un inhibidor del transporte de tricarboxilatos. Los resultados sugieren que a concentraciones de 1 mM de DCA y de 5 mM de AOA, BM y BT son suficientes para evaluar efectos metabólicos en células cerebrales durante el periodo perinatal. |
| metadata.dc.source: | NOVA Biomedical Sciences Journal; Vol. 3, Núm. 3 (2005); 56-67 Nova; Vol. 3, Núm. 3 (2005); 56-67 NOVA Ciências Biomédicas Publicação; Vol. 3, Núm. 3 (2005); 56-67 2462-9448 1794-2470 |
| URI: | https://repository.unad.edu.co/handle/10596/29806 |
| Other Identifiers: | http://hemeroteca.unad.edu.co/index.php/nova/article/view/19 10.22490/24629448.19 |
| Appears in Collections: | Revista Nova |
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