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Title:	Effect of Mercurius-Heel®s on the cytotoxicity of human gingival fibroblasts in an in vitro model Efecto del Mercurius-Heel®s sobre la citotoxicidad de fibroblastos gingivales humanos en un modelo in vitro
metadata.dc.creator:	Rueda, Gabriela García, Dabeiba Adriana Patiño, Liliana Catherine Cagueñas, Angélica García, Mariana
Keywords:	cytotoxicity; gingival fibroblasts; Mercurius Heel ®S; viability.;citotoxicidad; fibroblastos gingivales; Mercurius Heel ®S; viabilidad.
Publisher:	Universidad Colegio Mayor de Cundinamarca
metadata.dc.relation:	http://hemeroteca.unad.edu.co/index.php/nova/article/view/1027/1025 /*ref*/Chen FM, Jin Y. Periodontal tissue engineering and regeneration: current approaches and expanding opportunities. Tissue Eng Part B Rev 2010; 16: 219-255. /*ref*/Fayad I, Lafaurie G, Contreras A, Castillo D, Barón A, Aya M. Microflora subgingival en periodontitis crónica y agresiva en Bogotá, Colombia: un acercamiento epidemiológico. Biomédica 2007;27:21-33. /*ref*/Herrera D, Contreras A, Gamonal J, Oteo A, Jaramillo A, Silva N, Sanz M, Botero JE, León R. Subgingival microbial profiles in chronic periodontitis patients fron Chile, Colombia and Spain. J Clin Periodontol 2008;35: 106-113. /*ref*/Shaw D. Unethical aspects of homeopathic dentistry. Br Dent J. 2010;209:493-6. /*ref*/Rutten AL. Comparison of effectiveness of frequently and infrequently used homeopathic medicines. Homeopathy. 2011;100:175-82. /*ref*/Clausen J, van Wijk R, Albrecht H. Review of the use of high potencies in basic research on homeopathy. Homeopathy. 2011;100:288-92. /*ref*/Novella S, Roy R, Marcus D, Bell IR, Davidovitch N, Saine A. A debate: homeopathy--quackery or a key to the future of medicine? J Altern Complement Med 2008;14: 9-15. /*ref*/Gold PW, Novella S, Roy R, Marcus D, Bell I, Davidovitch N, Saine A. Homeopathy--quackery or a key to the future of medicine? Homeopathy 2008;97:28-33. /*ref*/Eames S, Darby P. Homeopathy and its ethical use in dentistry. Br Dent J. 2011;210:299-301. /*ref*/Mathie RT, Farrer S. Outcomes from homeopathic prescribing in dental practice: a prospective, research-targeted, pilot study. Homeopathy. 2007;96:74-81. /*ref*/Kaziro GS. Metronidazole (Flagyl) and Arnica Montana in the prevention of post-surgical complications, a comparative placebo controlled clinical trial. Br J Oral Maxillofac Surg. 1984;22:42-9. /*ref*/Lökken P, Straumsheim PA, Tveiten D, Skjelbred P, Borchgrevink CF. Effect of homoeopathy on pain and other events after acute trauma: placebo controlled trial with bilateral oral surgery. BMJ. 1995;310:1439-42. /*ref*/Haila S, Koskinen A, Tenovuo J. Effects of homeopathic treatment on salivary flow rate and subjective symptoms in patients with oral dryness: a randomized trial. Homeopathy. 2005;94:175-81. /*ref*/Farrer S, Baitson ES, Gedah L, Norman C, Darby P, Mathie RT. Homeopathic prescribing for chronic and acute periodontal conditions in 3 dental practices in the UK. Homeopathy. En prensa. /*ref*/Sinha MN, Siddiqui VA, Nayak C, Singh V, Dixit R, Dewan D, Mishra A. Randomized controlled pilot study to compare Homeopathy and Conventional therapy in Acute Otitis Media. Homeopathy. 2012;101:5-12. /*ref*/Nayak C, Singh V, Singh VP, Oberai P, Roja V, Shitanshu SS, Sinha MN, Deewan D, Lakhera BC, Ramteke S, Kaushik S, Sarkar S, Mandal NR, Mohanan PG, Singh JR, Biswas S, Mathew G. Homeopathy in chronic sinusitis: a prospective multi-centric observational study. Homeopathy. 2012;101:84-91. /*ref*/Mousavi F, Mojaver YN, Asadzadeh M, Mirzazadeh M. Homeopathic treatment of minor aphthous ulcer: a randomized, placebo-controlled clinical trial. Homeopathy. 2009;98:137-41. /*ref*/De Oliveira SM, de Oliveira CC, Abud AP, Guimarães Fde S, Di Bernardi RP, Coletto EL, Buchi Dde F. Mercurius solubilis: actions on macrophages. Homeopathy. 2011;100:228-36. /*ref*/Almirantis Y. Homeopathy--between tradition and modern science: remedies as carriers of significance. Homeopathy. 2013;102:114-22. /*ref*/de Araújo FR, de Castro CM, Severo MS, Diniz Mde F, Viana MT, Evêncio LB. Effect of Mercurius solubilis on the bacteriological response in the alveolitis process in rats. Homeopathy. 2009; 98:160-4.
metadata.dc.format.*:	application/pdf
metadata.dc.type:	info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion info:eu-repo/article/published info:eu-repo/article/published info:eu-repo/article/published
Description:	Periodontal disease is pathology of infectious origin, characterized because it causes destructive consequences to the supporting tissue of the tooth, its treatment is aimed at the destruction of the etiologic agents and to the periodontal regeneration. An alternative is the use of homeo-pathic agents because they are natural and are managed to very low concentrations, one of them is the Mercurius Heel ® S as an adjunctive therapy in infectious diseases. In this work, we present the results of an investigation whose objective was to evaluate the effect of Mercurius Heel® S on the viability of human gingival fibroblasts, which were undergoing treatment with Mercurius Heel® S for 15 minutes and two hours to concentrations from 300mg to 0.00006mg. After these times, the treatment was removed and the cells were maintained for 24, 48, and 72 more hours. It was followed by a colorimetric assay for cell viability and proliferation called MTS of Promega ®.Human gingival fibroblasts treated with Mercurius Heel® S showed an increase in cell prolife-ration compared with the untreated cells. Low medication concentrations of 0.0001 mg and 0.00006 mg showed a greater proliferation showed greater statistically differences. Finally, the effect of Mercurius Heel® was maintained for the first 48 hours. Considering the above, the Mercurius Heel® did not provide any cytotoxic effect on human gingival fibroblasts; on the contrary, the cells proliferated, suggesting its usefulness as supplementary treatment for periodontal disease. La enfermedad periodontal es una patología de origen infeccioso, caracterizada por ocasionar secuelas destructivas al tejido de soporte del diente, y cuyo tratamiento va encaminado a la destrucción de los agentes etiológicos y a la regeneración periodontal. Una alternativa es el uso de agentes homeopáticos ya que son naturales y se administran a muy bajas concentraciones, uno de ellos es el Mercurius Heel® S como coadyuvante en enfermedades infecciosas. En este trabajo se presentan los resultados de una investigación cuyo objetivo fue evaluar el efecto del Mercurius Heel® S en la viabilidad de fibroblastos gingivales humanos, que fueron sometidos a tratamiento con Mercurius Heel® S durante 15 minutos y dos horas a concentraciones desde 300mg hasta 0.00006mg. Transcurridos esos tiempos, se retiró el tratamiento y las células fueron mantenidas durante 24, 48 y 72 horas más. Posteriormente se realizó un ensayo colorimétrico de viabilidad y proliferación celular denominado MTS de promega®Los fibroblastos gingivales humanos tratados con Mercurius Heel® S mostraron un aumento en la proliferación celular comparada con las células no tratadas. Bajas concentraciones del medicamento 0,0001mg y 0,00006mg mostraron una mayor proliferación observando diferencias estadísticamente significativas. El tratamiento a 15 minutos mostró mejores resultados con respecto al tratamiento de 2 horas con diferencias estadísticamente significativas también. Finalmente el efecto del Mercurius Heel® se mantuvo hasta las primeras 48 horas. Considerando lo anterior, el Mercurius Heel® no presentó ningún efecto citotóxico en los fibroblastos gingivales; por el contrario, las células proliferaron, lo que sugiere su utilidad como tratamiento complementario en la enfermedad periodontal.
metadata.dc.source:	NOVA Biomedical Sciences Journal; Vol. 11, Núm. 20 (2013); 15-22 Nova; Vol. 11, Núm. 20 (2013); 15-22 NOVA Ciências Biomédicas Publicação; Vol. 11, Núm. 20 (2013); 15-22 2462-9448 1794-2470
URI:	https://repository.unad.edu.co/handle/10596/29891
Other Identifiers:	http://hemeroteca.unad.edu.co/index.php/nova/article/view/1027 10.22490/24629448.1027
Appears in Collections:	Revista Nova

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