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Title:	Evaluation of bacterial freeze native species conservation Evaluación de la congelación para conservación de especies autóctonas bacterianas
metadata.dc.creator:	Corrales Ramírez MSC, Lucía Constanza Sánchez Leal MSC, Ligia Consuelo
Keywords:	Ciencias Naturales,Ciencias Biológicas,Biología Celular y Microbiología;Congelación; descongelación; viabilidad bacteriana; actividad metabólica;freezing; defrost; viability; metabolic activity.
Publisher:	Universidad Colegio Mayor de Cundinamarca
metadata.dc.relation:	http://hemeroteca.unad.edu.co/index.php/nova/article/view/333/1208 /*ref*/Tangue P, Van D, Thevelein J. Determinants of freeze tolerance in microorganisms, physiological importance and biotechnological applications. Advances in applied Microbiology 2003;53:129 . 176. /*ref*/Dumont F, Marechal P, Gervais P. Influence of cooling rateo in Saccharomyces cerevisiae destruction during freezing: unexpected viability at ultra-rapid cooling rates. Criobiology 2003; 46: 33-42. /*ref*/Ham B, Bischof J. Direct cell injury associated with eutectic crystallyzation during freezing. Criobiology 2004; 48: 8-21. /*ref*/Wowk B, Fahy G. Inhibition of bacterial ice nucleation by polyglicerol polymers Volume. Criobiology 2002; 44:14-23. 29 /*ref*/Acker J, Locksley E. Protective effect of intracellular ice during freezing. Criobiology 2003; 46: 197-202. /*ref*/Hubalek Z. Review. Protectants used in the cryopreservation of microorganisms. Criobiology 2003; 46: 205-229. /*ref*/Aulet De Saab O. y otros. Comparative Study of Preservation and Storage of Haemophilus influenzae Memórias do Instituto Oswaldo Cruz 2001;96(4). /*ref*/Mukamolova G, Kaprelyants A, Kell D, Young M. Adoption of the transiently non-culturable state a bacterial survival strategy. Advances in applied Microbiology 2003;47:65 . 129. /*ref*/Wang Jung Hui. A comprehensive evaluation of the effects and mechanisms of antifreeze proteins during low temperature preservation. Criobiology 2000; 41: 1 - 9. /*ref*/Charalambous B. M., y colaboradores. Quantitative Validation of Media for Transportation and Storage of Streptococcus pneumoniae. Journal of Clinical Microbiology 2003; 41(12):5551-5556. /*ref*/Siberry George, Brahmadathan K.N. et al. Comparison of different culture media and storage temperatures for the longterm preservation of Streptococcus pneumoniae in the tropics. Bulletin of the world health organization 2001; 79: 43-47
metadata.dc.format.*:	application/pdf
metadata.dc.type:	info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion article article article
Description:	The bacterial microorganisms constitute a good part of the biodiversity of our planet and the importance of its knowledge and preservation has been increased as their multiple utilities in different fields are discovered. The conservation can be done by different methods and one of which offers better results is the freezing; for that reason east project was made whose objective was to evaluate the effects of the freezing in 18 native bacterial species of the collection of cultures of the Program of Bacteriology of the University Colegio Mayor de Cundinamarca, Bogotá- Colombia. The microorganisms including in the study, they were selected considering the variability of metabolic activity between species and their clinical importance, industrialist, biotechnological or environmental. The obtained results were similar in the analyzed species and although some had difficulty to recover their viability and metabolic activity in the first sowing, later they presented displayed one better answer, what allowed to conclude that the phenotypic and genotypic stability does not seem to depend on means of culture nor on the used preservation substance, but of the procedures of freezing and defrost with which the experiment was made. The recommendation of the investigating group is to establish own standardizations and protocols that guarantee the viability and biochemical stability of the microorganisms that are conserved by this method. Los microorganismos bacterianos constituyen una buena parte de la biodiversidad de nuestro planeta y la importancia de su conocimiento y preservación se ha incrementado a medida que se descubren sus múltiples utilidades en diferentes campos. La conservación se puede hacer por diferentes métodos y uno de los que brinda mejores resultados es la congelación; por ello se realizó este proyecto cuyo objetivo fue evaluar los efectos de la congelación en 18 especies bacterianas autóctonas de la colección de cultivos del Programa de Bacteriología de la Universidad Colegio Mayor de Cundinamarca, Bogotá- Colombia. Los microorganismos incluidos en el estudio, se seleccionaron teniendo en cuenta la variabilidad de actividad metabólica entre especies y su importancia clínica, industrial, biotecnológica o ambiental. Los resultados obtenidos fueron similares en las especies analizadas y aunque en algunas se observó dificultad para recuperar su viabilidad y actividad metabólica en la primera siembra, después de esta presentaron una mejor respuesta; lo que permitió concluir que la estabilidad fenotípica y genotípica de los microorganismos estudiados, no parece depender del medio de cultivo ni del conservante utilizado, sino de los procedimientos de congelación y descongelación con los cuales se realiza el experimento. La recomendación del grupo investigador es establecer estandarizaciones y protocolos propios que garanticen la viabilidad y estabilidad bioquímica de los microorganismos que se conserven por este método.
metadata.dc.source:	NOVA Biomedical Sciences Journal; Vol. 3, Núm. 4 (2005); 21-29 Nova; Vol. 3, Núm. 4 (2005); 21-29 NOVA Ciências Biomédicas Publicação; Vol. 3, Núm. 4 (2005); 21-29 2462-9448 1794-2470
URI:	https://repository.unad.edu.co/handle/10596/30053
Other Identifiers:	http://hemeroteca.unad.edu.co/index.php/nova/article/view/333 10.22490/24629448.333
Appears in Collections:	Revista Nova

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