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https://repository.unad.edu.co/handle/10596/46982| Title: | Antimicrobial potential of plant extracts and their mixtures against bacteria associated with conjunctivitis Potencial antimicrobiano de extractos de plantas medicinales y sus mezclas frente a bacterias asociadas con conjuntivitis |
| metadata.dc.creator: | Velasco Garcia, Wendy Johanna Pabón Baquero, Ludy C. Hernández-Rodríguez, Patricia |
| Keywords: | conjunctivitis;antibacterial activity;plant extracts;staphylococcus;infection;conjuntivitis;actividad antimicrobiana;extractos de plantas;staphylococcus;infecciones |
| Publisher: | Universidad Colegio Mayor de Cundinamarca |
| metadata.dc.relation: | https://hemeroteca.unad.edu.co/index.php/nova/article/view/5294/5085 |
| metadata.dc.format.*: | application/pdf |
| metadata.dc.type: | info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion info:eu-repo/article/published info:eu-repo/article/published info:eu-repo/article/published |
| Description: | Introduction. Bacterial conjunctivitis is one of the ocular infections with the highest rate of ophthalmic consultation, being the Staphylococcus genus the etiological agent that presents the greatest resistance to antibiotics. Objective. To determine the antimicrobial potential of medicinal plant extracts and their mixtures against bacteria associated with conjunctivitis. Methods. From plants such as Belladonna, Calendula, Basil, Achiote and Romerillo, ethanolic extracts were prepared and their antibacterial potential against S. aureus and S. epidermidis was evaluated by means of disk diffusion and tube dilution techniques. The effect of the mixtures was determined for the extract with the best activity and the antibiotic with the highest inhibition halo through the board technique. Results. It was found that the extract with the highest potential for S. aureus was that of annatto (leaves) with an inhibition halo of 13 mm and MICs of 15 mg / mL, while for S. epidermidis it was those of annatto leaves and fruits with halos of 16.6 and 9.6 mm and MICs of 15 and 30 mg / mL respectively. In relation to the combinations, the annatto extract and ciprofloxacin showed a partial synergistic effect for S. aureus with a CFI of 0.83 and an additive effect for S. epidermidis with a CFI of 1.84. Conclusions. This work constitutes the basis for futureresearch aimed at the development of bioproducts for ocular use that can be considered as an alternative in the treatment of infections caused by Staphylococcus. Introducción. La conjuntivitis bacteriana es una de las infecciones oculares con mayor tasa de consulta oftálmica, siendo el género Staphylococcus el agente etiológico que presenta mayor resistencia a los antibióticos. Objetivo. Determinar el potencial antimicrobiano de extractos de plantas medicinales y sus mezclas frente a bacterias asociadas con conjuntivitis. Métodos. A partir de plantas como Belladona, Caléndula, Albahaca, Achiote y Romerillo se prepararon los extractos etanólicos y se evaluó su potencial antibacteriano frente a S. aureus y S. epidermidis, mediante las técnicas de difusión en disco y dilución en tubo. El efecto de las mezclas se determinó para el extracto con mejor actividad y el antibiótico con mayor halo de inhibición a través de la técnica de tablero. Resultados. Se encontró que el extracto con mayor potencial para S. aureus fue el de achiote (hojas) con un halo de inhibición de 13 mm y CMIs de 15 mg/mL, mientras que para S. epidermidis fueron los de hojas y frutos de achiote con halos de 16,6 y 9,6 mm y CMIs de 15 y 30 mg/mL respectivamente. En relación con las combinaciones, el extracto de achiote y ciprofloxacina mostró un efecto de sinergia parcial para S. aureus con una CFI de 0,83 y un efecto aditivo para S. epidermidis con una CFI de 1,84. Conclusiones. Este trabajo se constituye en la base de futuras investigaciones orientadas hacia el desarrollo de bioproductos de uso ocular que puedan ser considerados como alternativa en el tratamiento de infecciones causadas por Staphylococcus. |
| metadata.dc.source: | NOVA Biomedical Sciences Journal; Vol. 19 No. 36 (2021); 95 - 108 Nova; Vol. 19 Núm. 36 (2021); 95 - 108 NOVA Ciências Biomédicas Publicação; v. 19 n. 36 (2021); 95 - 108 2462-9448 1794-2470 |
| URI: | https://repository.unad.edu.co/handle/10596/46982 |
| Other Identifiers: | https://hemeroteca.unad.edu.co/index.php/nova/article/view/5294 10.22490/24629448.5294 |
| Appears in Collections: | Revista Nova |
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