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| DC Field | Value | Language |
|---|---|---|
| dc.creator | Mora López, Marcela | en |
| dc.creator | Ochoa Aristizabal, Ana Maria | en |
| dc.creator | Osorio Echeverri, Victor Manuel | en |
| dc.creator | Mora López, Marcela | es |
| dc.creator | Ochoa Aristizabal, Ana Maria | es |
| dc.creator | Osorio Echeverri, Victor Manuel | es |
| dc.date | 2024-06-14 | - |
| dc.identifier | https://hemeroteca.unad.edu.co/index.php/riaa/article/view/6789 | - |
| dc.identifier | 10.22490/21456453.6789 | - |
| dc.description | Contextualization: The production of cape gooseberry (Physalis peruviana L.), an exotic fruit of great commercial importance in Colombia, has been limited by the presence of Fusarium sp. Which causes losses of up to 40% of production; this pathogen is mainly controlled with chemical synthesis products. Knowledge gap: The individual use of microorganisms for biological control has become a useful strategy to reduce the impact caused by Fusarium sp. However, its activity is limited in the field, and therefore it is necessary to carry out consortium combinations of different native microbial genera as a promising tool for biological control. Purpose: To evaluate the antagonistic activity of the combined application of Trichoderma sp. and Bacillus sp. as biocontrollers of Fusarium sp. at greenhouse level in cape gooseberry (Physalis peruviana L.) Methodology: Microorganisms were isolated from soil samples and plant material from a cape gooseberry crop. Isolates consistent with Bacillus sp. and Trichoderma sp. were confronted in vitro conditions to determine the percentage of inhibition over three Fusarium sp. that showed pathogenicity in cape gooseberry seeds according to their germination and root length. The in vivo antagonist activity was tested individually and in a consortium of Bacillus sp. and Trichoderma sp. that most inhibited Fusarium sp. with a higher pathogenicity index. Additionally, these isolates were identified by morphological, molecular and phylogenetic techniques. Results and conclusions: Seven native isolates consistent with Bacillus sp. with inhibitory activity against three Fusarium sp. were obtained. The bacterial isolate IB4B (identified as Bacillus sp.) registered the highest percentage of inhibition (48%), and TV1 was the fungus with the highest activity (64%) although. It did not differ statistically from TV2 (identified as Trichoderma asperellum). The pathogenicity test indicated that UCMV21 (identified as Fusarium oxysporum) inhibited the germination of 58% of the evaluated seeds. In the in vivo tests on cape gooseberry seedlings, UCMV21 presented low pathogenicity by developing few symptoms of vascular wilt. However, treatments with the Bacillus sp. and Trichoderma sp., achieved control of the pathogen and improved the root length of the seedlings. | en |
| dc.description | Contextualización: La producción de la uchuva (Physalis peruviana L.), una fruta exótica de gran importancia comercial en Colombia; se ha visto limitada por la presencia de Fusarium sp. que causa pérdidas de hasta el 40% de la producción; este patógeno se controla, principalmente, con productos de síntesis química. Vacío de conocimiento: El uso individual de microorganismos para el control biológico se ha convertido en una estrategia útil para disminuir el impacto causado por Fusarium sp. Sin embargo, su actividad se encuentra limitada en campo y por lo tanto es necesario realizar combinaciones en consorcio de diferentes géneros microbianos nativos como herramienta promisoria para el control biológico. Objetivos: Evaluar la actividad antagónica de la aplicación combinada de Trichoderma sp. y Bacillus sp. como biocontroladores de Fusarium sp. a nivel de invernadero en uchuva (Physalis peruviana L.) Metodología: Se realizó el aislamiento de microorganismos de muestras de suelo y material vegetal de un cultivo de uchuva. Los aislados consistentes con Bacillus sp. y Trichoderma sp. fueron enfrentados en condiciones in vitro para determinar el porcentaje de inhibición sobre tres Fusarium sp. que mostraron patogenicidad en semillas de uchuva según su germinación y longitud radicular. La actividad antagonista in vivo se probó de manera individual y en consorcio de Bacillus sp. y Trichoderma sp. que más inhibieron a Fusarium sp. con mayor índice de patogenicidad, adicionalmente, estos fueron identificados por técnicas morfológicas, moleculares y filogenéticos. Resultados y conclusiones: Se obtuvieron siete aislados nativos consistentes con Bacillus sp. con actividad inhibitoria contra tres Fusarium sp. El aislado bacteriano IB4B (identificado como Bacillus sp.) registró el mayor porcentaje de inhibición (48%), y TV1 fue el hongo con mayor actividad (64%), aunque no difiere estadísticamente de TV2 (identificado como Trichoderma asperellum). La prueba de patogenicidad indicó que UCMV21 (identificado como Fusarium oxysporum) inhibió la germinación del 58% de las semillas evaluadas. En pruebas in vivo en plántulas de uchuva, UCMV21 presentó baja patogenicidad al desarrollar pocos síntomas de marchitez vascular, sin embargo, los tratamientos con el consorcio de Bacillus sp. y Trichoderma sp., lograron el control del patógeno y mejoraron la longitud radicular de las plántulas. | es |
| dc.format | application/pdf | - |
| dc.format | application/zip | - |
| dc.language | spa | - |
| dc.publisher | Sello Editorial UNAD | es |
| dc.relation | https://hemeroteca.unad.edu.co/index.php/riaa/article/view/6789/7037 | - |
| dc.relation | https://hemeroteca.unad.edu.co/index.php/riaa/article/view/6789/7333 | - |
| dc.relation | https://hemeroteca.unad.edu.co/index.php/riaa/article/view/6789/7335 | - |
| dc.relation | https://hemeroteca.unad.edu.co/index.php/riaa/article/view/6789/7336 | - |
| dc.relation | https://hemeroteca.unad.edu.co/index.php/riaa/article/view/6789/7334 | - |
| dc.rights | Derechos de autor 2024 Revista de Investigación Agraria y Ambiental | es |
| dc.rights | http://creativecommons.org/licenses/by-nc-sa/4.0 | es |
| dc.source | Revista de Investigación Agraria y Ambiental; Vol. 15 No. 2 (2024); 35-67 | en |
| dc.source | Revista de Investigación Agraria y Ambiental; Vol. 15 Núm. 2 (2024); 35-67 | es |
| dc.source | 2145-6453 | - |
| dc.source | 2145-6097 | - |
| dc.subject | Biocontrol | en |
| dc.subject | Biotechnology | en |
| dc.subject | Inhibition | en |
| dc.subject | Integrad pest control | en |
| dc.subject | Phytopathology | en |
| dc.subject | Biocontrol | es |
| dc.subject | Biotecnología | es |
| dc.subject | Inhibición | es |
| dc.subject | Control integrado de plagas | es |
| dc.subject | Fitopatología | es |
| dc.title | Biological control of Fusarium sp. in cape gooseberry plants (Physalis peruviana L.) at greenhouse level by combined application of Trichoderma sp. AND Bacillus sp. | en |
| dc.title | Control biológico contra Fusarium sp. en plantas de uchuva (Physalis peruviana L.) a nivel de invernadero mediante aplicación combinada de Trichoderma sp. y Bacillus sp. | es |
| dc.type | info:eu-repo/semantics/article | - |
| dc.type | info:eu-repo/semantics/publishedVersion | - |
| dc.type | Área Agrícola | es |
| Appears in Collections: | Revista RIAA | |
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