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Generalidades de la producción de embriones bovinos in vitro

dc.creatorGonella Diaza, Ángela María
dc.creatorAtuesta Bustos, Jorge Eduardo
dc.creatorBernal Ulloa, Sandra Milena
dc.creatorChacón Jaramillo, Liliana
dc.date2013-01-15
dc.date.accessioned2019-11-08T21:21:31Z
dc.date.available2019-11-08T21:21:31Z
dc.identifierhttp://hemeroteca.unad.edu.co/index.php/riaa/article/view/1967
dc.identifier10.22490/21456453.1967
dc.identifier.urihttps://repository.unad.edu.co/handle/10596/29358
dc.descriptionLa producción de embriones in vitro (PIV) es una biotecnología reproductiva que impacta los sistemas ganaderos favoreciendo el mejoramiento genético y que también se ha utilizado como modelo de investigación del desarrollo embrionario en otras especies. Para el desarrollo de ésta técnica, se colectan oocitos in vivo por medio de laparoscopia y aspiración folicular guiada por ultrasonografía ó a través de ovarios recolectados en plantas de sacrificio. Los oocitos son sometidos a procesos de maduración in vitro, utilizando diversos medios, adición de gonadotropinas y factores de crecimiento. Los espermatozoides son sometidos a tratamientos de capacitación espermática in vitro, donde adquieren capacidad fertilizante y se separan los espermatozoides vivos de los componentes seminales y crio-protectores. Para la fertilización in vitro se realiza un cocultivo de espermatozoides y oocitos en un medio que favorece la actividad espermática y penetración de la zona pelúcida. Posteriormente, los embriones obtenidos son sometidos a cultivo en medios que contengan fuentes de energía, aminoácidos y factores de crecimiento, con el fin de mantener su sobrevivencia y desarrollo. En la actualidad la técnica de PIV en bovinos se ha masificado y se trabaja en los procesos de estandarización en otras especies. A partir de la PIV se dio el desarrollo de nuevas biotecnologías como la clonación y transgénesis. El objetivo de esta revisión es describir las fases de la PIV y su relación con los eventos fisiológicos de la fertilización y desarrollo embrionario temprano.es-ES
dc.formatapplication/pdf
dc.languagespa
dc.publisherUniversidad Nacional Abierta y a Distancia, UNADes-ES
dc.relationhttp://hemeroteca.unad.edu.co/index.php/riaa/article/view/1967/2188
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dc.rightsCopyright (c) 2017 Revista de Investigación Agraria y Ambientales-ES
dc.rightshttp://creativecommons.org/licenses/by-nc-sa/4.0es-ES
dc.sourceRevista de Investigación Agraria y Ambiental; Vol. 4, Núm. 1 (2013); 65 - 80en-US
dc.sourceRevista de Investigación Agraria y Ambiental; Vol. 4, Núm. 1 (2013); 65 - 80es-ES
dc.source2145-6453
dc.source2145-6097
dc.subjectbiotecnología, desarrollo embrionario, espermatozoides, fertilización, oocitoses-ES
dc.titleGeneralidades de la producción de embriones bovinos in vitroes-ES
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion


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