Influence of the Carbon / Nitrogen Ratio in the Production of Total Proteins in Chlorella Vulgaris UTE X 1803.

Durán Serrano, Linda Estephany; Barajas Solano, Andrés Fernando; González Delgado, Ángel Darío; Barajas Ferreira, Crisóstomo

dc.creator	Durán Serrano, Linda Estephany
dc.creator	Barajas Solano, Andrés Fernando
dc.creator	González Delgado, Ángel Darío
dc.creator	Barajas Ferreira, Crisóstomo
dc.date	2012-06-02
dc.date.accessioned	2019-11-08T21:23:34Z
dc.date.available	2019-11-08T21:23:34Z
dc.identifier	http://hemeroteca.unad.edu.co/index.php/publicaciones-e-investigacion/article/view/1111
dc.identifier	10.22490/25394088.1111
dc.identifier.uri	https://repository.unad.edu.co/handle/10596/29686
dc.description	Microalgae represent a source of protein with potential applications in human and animal nutrition, since they possess a good balance of amino-acids and low content of nucleic acids in comparison with other sources of protein. However, the low productivity of cultures has prevented production at a large scale. In this work, we studied the total production of protein in Chlorella vulgaris at a laboratory scale, analyzing different pre-established parameters under the carbon/nitrogen ratio. For this, we performed mixotrophic cultures during 5 days at different concentrations of acetate (5Mm, 10Mm, 20Mm) and sodium nitrate (0,97Mm, 1,94Mm, 2,94Mm). All treatments were kept at 23 ± 1ºC, with light/dark cycles of 12h: 12h for 5 days. The highest value of protein concentration was obtained from the culture R9 with a production of 0,78±0.18 g/L, 1.7 times higher than the production of the control culture treatment. (0,46 g/L). R9 was also the culture that consumed the most nitrogen (91%). Based on these results and their analysis, we found that the total content of protein in C. vulgaris is directly proportional to the amount of acetate and nitrate present in the culture media, since the algae grown in cultures where these nutrients were limited exhibited the lowest values in terms of protein concentration	en-US
dc.description	Las microalgas representan una fuente de proteína con posibles aplicaciones en la nutrición humana y animal ya que poseen un buen balance de aminoácidos y bajos valores de ácidos nucleícos, en comparación con otras fuentes de proteína; sin embargo, la baja productividad de estos cultivos ha impedido implementar una producción a gran escala. En este trabajo se estudió la influencia de la relación carbono/nitrógeno en la productividad de proteínas totales presentes en Chlorella vulgarisa escala de laboratorio. Se efectuaron cultivos mixotróficos durante 5 días con diferentes concentraciones de acetato (5mM, 10mM, 20mM), y nitrato de sodio (0,97mM, 1,94mM, 2,94mM). Todos los tratamientos se realizaron a 23 ± 1ºC, con ciclos luz-oscuridad 12h: 12h. El valor más alto de concentración de proteínas se obtuvo en el medio de cultivo R9 con una productividad de 0,78±0.18 g/L, superando 1,7 veces más en producción, al cultivo control (0,46 g/L). De igual manera, fue el cultivo que consumió mayor cantidad de nitrógeno (91%). Con base en los datos obtenidos y el análisis de los mismos, se encontró que el contenido de proteínas totales en C. vulgaris es directamente proporcional a la cantidad de acetato y nitrato presentes en el medio, mientras que la respuesta de la cepa algal en cultivos con limitación de estos nutrientes, registró los valores más bajos en relación con la concentración de proteínas.	es-ES
dc.format	application/pdf
dc.language	spa
dc.publisher	Universidad Nacional Abierta y a Distancia, UNAD	es-ES
dc.relation	http://hemeroteca.unad.edu.co/index.php/publicaciones-e-investigacion/article/view/1111/1275
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dc.rights	Copyright (c) 2015 Publicaciones e Investigación	es-ES
dc.rights	http://creativecommons.org/licenses/by-sa/4.0	es-ES
dc.source	Magazine specialized in Engineering; Vol. 6 (2012); 49-58	en-US
dc.source	Publicaciones e Investigación; Vol. 6 (2012); 49-58	es-ES
dc.source	2539-4088
dc.source	1900-6608
dc.subject	acetate; microalgae; mixotrophic culture; sodium nitrate; productivity	en-US
dc.subject	acetato; cultivos mixotróficos; microalgas; nitrato de sodio; productividad	es-ES
dc.title	Influence of the Carbon / Nitrogen Ratio in the Production of Total Proteins in Chlorella Vulgaris UTE X 1803.	en-US
dc.title	Influencia de la Relación Carbono/Nitrógeno en la Producción de Proteínas Totales en Chlorella Vulgari s UTE X 1803.	es-ES
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dc.type	info:eu-repo/semantics/publishedVersion