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Assessment of Morphologic Characteristics and Immunophenotype of Mesenchimal Stem Cell Culture Obtained From Umbilical Cord Blood and Bone Marrow

dc.creatorArévalo Romero, Jenny Andrea
dc.creatorPáez Guerrero, Diana
dc.creatorRodríguez Pardo, Viviana Marcela
dc.descriptionBone marrow and umbilical cord blood are sources of mesenchymal stem cells. They are important for tissue regeneration and immunomodulation. The object of this work was to isolate and culture mesenchymal stem cells from bone marrow and umbilical cord blood cells. Bone marrow samples were obtained from patients at the Orthopedic Service of the San Ignacio Hospital and cord blood samples were obtained from women with normal pregnancy at the Gineco-Obstetrics Service of the Occidente de Kennedy Hospital in Bogotá, Colombia. We determined cellular rate generation, morphologic characteristics and antigens expression for cytometry flow.Efficiency isolation was 30% for mesenchymal stem cells from umbilical cord blood and 100% for bone marrow, with cellular rate generation between 20 and 50 minutes and 16 and 39 minutes respectively. Morphologic differences were determined with microscopy and Wright coloration. During primary culture, 3.54% of CD34+/CD45+ was observed, but after 10 days, they were at 0.19% (CD34+/CD45+). Isolation of mesenchymal stem cells from bone marrow is efficient. There are differences between mesenchymal stem cells from umbilical cord blood and bone marrow. CD105 antigen is an important marker for mesenchymal cells lineage.en-US
dc.descriptionLa sangre del cordón umbilical y la médula ósea humana son una alternativa para el aislamiento y cultivo de células madre mesenquimales, útiles en terapias de regeneración tisular e inmunomodulación. El objetivo de este trabajo fue aislar y cultivar células madre mesenquimales a partir de la sangre del cordón umbilical y de la médula ósea. Se recolectaron muestras de sangre de cordón umbilical en el servicio de Gineco-Obstetricia del Hospital Occidente de Kennedy en Bogotá, Colombia. La recolección de médula ósea se realizó en los servicios de ortopedia y traumatología del hospital universitario San Ignacio de Bogotá, Colombia. Se evaluó la tasa de generación celular, características morfológicas por microscopia invertida y tinción de Wright, asi como el inmunofenotipo de las poblaciones celulares por citometría de flujo.La eficiencia de aislamiento de las células madre mesenquimales a partir de sangre de cordón umbilical fue del 30% con una tasa de generación entre 20 y 50 minutos. A partir de médula ósea el aislamiento fue del 100%, con un tiempo de generación entre 16 y 39 minutos. Se observaron diferencias morfológicas por tinción de Wright y la presencia de progenitores hematopoyéticos durante el cultivo primario (3.54% de CD34+/CD45+), que disminuían cuando se realizaba el primer pase del cultivo (0.19% de CD34+/CD45+). El aislamiento de células madre mesenquimales es más eficiente a partir de medula ósea.Se observan diferencias morfológicas por microscopia invertida y tinción de Wright entre células aisladas de sangre de cordón umbilical y médula ósea. El antígeno CD105 constituye un marcador importante en el establecimiento del perfil inmunofenotípico de células madre
dc.publisherUniversidad Colegio Mayor de Cundinamarcaes-ES
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dc.rightsCopyright (c) 2007 NOVA Publicación en Ciencias Biomédicases-ES
dc.sourceNOVA Biomedical Sciences Journal; Vol. 5, Núm. 8 (2007); 114-126en-US
dc.sourceNova; Vol. 5, Núm. 8 (2007); 114-126es-ES
dc.sourceNOVA Ciências Biomédicas Publicação; Vol. 5, Núm. 8 (2007); 114-126pt-BR
dc.subjectCiencias Médicas y de la Salud,Medicina Clínica,Otros Temas de Medicina Clínicaes-ES
dc.subjectcélulas madre; células mesenquimales; citometría de flujo; cordón umbilical; médula óseaes-ES
dc.subjectbone marrow; flow cytometry; mesenchymal cells; stem cells; umbilical cord blooden-US
dc.titleAssessment of Morphologic Characteristics and Immunophenotype of Mesenchimal Stem Cell Culture Obtained From Umbilical Cord Blood and Bone Marrowen-US
dc.titleEvaluación de características morfológicas e inmunofenotipo de células madre mesenquimales en cultivo obtenidas a partir de sangre de cordón umbilical y médula ó

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