Isolamento de lectinas por cromatografia de afinidade

Torres Romero, José Camilo; Ortega Torres, Myriam Janeth; Infante Betancour, Jhon Alexander; Maia Cavalcante, Cicero Antonio

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Torres Romero, José Camilo

Ortega Torres, Myriam Janeth

Infante Betancour, Jhon Alexander

Maia Cavalcante, Cicero Antonio

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Universidad Nacional Abierta y a Distancia, UNAD

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Abstract

Cromatografia de afinidade é uma técnica usada para separar compostos, como, por exemplo, determinadas proteínas, que têm a capacidade de se ligar não covalentemente e reversivelmente a moléculas específicas conhecidas como ligantes. Esse método difere das técnicas de cromatografia clássica devido a proteína conseguir ser separada com base em uma única propriedade bioquímica. Em cromatografia de afinidade, o ligante está ligado covalentemente à matriz, que deve ser quimicamente inerte, porosa e também ter uma variedade de grupos funcionais adequados para acoplamento com ligantes diferentes. Várias matrizes e ligantes são usados em cromatografia de afinidade, dependendo da proteína a ser purificada. Esse trabalho descreverá alguns aspectos importantes para o isolamento de lectinas através da técnica de cromatografia de afinidade utilizando-se carboidratos.

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http://hemeroteca.unad.edu.co/index.php/riaa/article/view/1837/2057
http://hemeroteca.unad.edu.co/index.php/riaa/article/view/1837/2219
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