Dendritic Cells From Human Monocytes: As a Tool to Study Antileishmanials Agents

Las células dendríticas son consideradas potentes células presentadoras de antígeno, fundamentales para el inicio y desarrollo de una respuesta inmunitaria efectiva frente a diversos agentes infecciosos. El estudio de la interacción parásito-células dendríticas, es esencial para la evaluación y desarrollo de nuevas estrategias terapéuticas profilácticas capaces de controlar de una manera segura y efectiva el agente patógeno, si se tiene en cuenta que diferentes agentes patógenos (ej: parásitos perteneciente al género Leishmania) pueden afectar la maduración y función de las células dendríticas, como uno de los principales mecanismos de evasión de la respuesta.En el presente estudio, nosotros determinamos que las células dendríticas derivadas de monocitos de sangre periférica humana diferenciadas luego de 72 horas de cultivo en presencia de interleuquina 4 y factor estimulador de colonias granulocito/macrófago, pueden ser utilizadas exitosamente para la evaluación de la actividad anti-leishmanial e inmuno-moduladora de nuevas formulaciones terapéuticas para el control de la leishmaniosis.

Dendritic cells, considered as powerful antigen presenting cells, are fundamental fir the beginning and development of an effective immune response against diverse infections agents. The study of interaction between dendritic cells and parasites is essential for the evaluation and development of new prophylactic and therapeutics strategies able to control the pathogenic agent in a safe and effective way. This is important when considering that different pathogenic agents (e.g. Leishmania parasites) can affect the maturation process and dendritic cells function, as an effective mechanism to evade the immune response.In this study, it was determined that dendritic cells differentiated from human peripheral blood monocytes (after 72 hours culture in presence of 4 interleukin and granulocyte/macrophage colonies stimulating factor) can successfully be used for evaluation of anti-leishmanial activity and immunomodulator effect of new therapeutics formulations for leishmaniasis control.

College

http://hemeroteca.unad.edu.co/index.php/nova/article/view/407/1146
/*ref*/WHO. Neglected tropical diseases, hidden successes, emerging opportunities 2006. 2. INS. (Ministerio de la Protección Social. Dirección General de Salud Pública Instituto Nacional de Salud. Subdirección de Vigilancia y Control en Salud Pública). Situacion de la leishmaniasis en Colombia, semanas epidemiologicas 1- 52 de 2007. 2008. 3. King RJ, Campbell-Lendrum DH, Davies CR. Predicting geographic variation in cutaneous leishmaniasis, Colombia. Emerg Infect Dis. 2004;10: 598-607. 4. Murray HW, Berman JD, Davies CR, Saravia NG. Advances in leishmaniasis. Lancet. 2005;366:1561–1577. 5. Moll H. Dendritic cells as a tool to combat infectious diseases. Immunol Lett. 2003;85:153-157. 6. Palucka K, Banchereau J. How dendritic cells and microbes interact to elicit or subvert protective immune responses. Curr Opin Immunol 2002;14:420–431. 7. Banchereau J, Steinman RM. Dendritic cells and the control of immunity. Nature. 1998;392:245-252. 8. Liu YJ, Kanzler H, Soumelis V, Gilliet M. Dendritic cell lineage, plasticity and cross-regulation. Nat Immunol. 2001;2:585-589. 9. Mellman I, Steinman RM. Dendritic cells: specialized and regulated antigen processing machines. Cell. 2001;106:255-258. 10. Liu YJ. Dendritic cell subsets and lineages, and their functions in innate and adaptive immunity. Cell. 2001;106:259-262. 11. Lipscomb MF, Masten BJ. Dendritic cells: immune regulators in health and disease. Physiol Rev. 2002;82:97-130. 12. Banchereau J, Briere F, Caux C, Davoust J, Lebecque S, Liu YJ, Pulendran B, Palucka K. Immunobiology of Dendritic Cells. Annu Rev Immunol. 2000;18:767–811. 13. Kapsenberg ML. Dendritic-cell control of pathogen-driven T-cell polarization. Nat Rev Immunol. 2003;3:984-993. 14. Curfs JH, Meis JF, Hoogkamp-Korstanje JA. A primer on cytokines: sources, receptors, effects, and inducers. Clin Microbiol Rev. 1997;10:742-780. 15. Leon B, Lopez-Bravo M, Ardavin C. Monocyte-derived dendritic cells. Semin Immunol. 2005;17:313 318. 16. Moll H. Dendritic cells and host resistance to infection. Cell Microbiol. 2003;5:493-500. 17. Scott P, Hunter CA. Dendritic cells and immunity to leishmaniasis and toxoplasmosis. Curr. Opin. Immunol. 2002;14:466–470. 18. Sacks D, Sher A. Evasion of innate immunity by parasitic protozoa. Nat Immunol. 2002;3:1041-1047. 19. Dauer M, Obermaier B, Herten J, Haerle C, Pohl K, Rothenfusser S, Schnurr M, Endres S, Eigler A. Mature dendritic cells derived from human monocytes within 48 hours: a novel strategy for dendritic cell differentiation from blood precursors. J Immunol. 2003;170:4069-4076. 20. Sallusto F, Lanzavecchia A. Efficient Presentation of Soluble Antigen by Cultured Human Dendritic Cells Is Maintained by Granulocyte/Macrophage Colony-stimulating Factor Plus Iuterleukin 4 and Downregulated by Tumor Necrosis Factor alfa. J Exp Med 1994;179:1109-1118. 21. Delgado G, Parra-López CA, Vargas LE, Hoya R, Estupiñán M, Guzmán F, Torres A y col. Characterizing cellular immune response to kinetoplastid membrane protein-11 (KMP-11) during Leishmania (Viannia) panamensis infection using dendritic cells (DCs) as antigen presenting cells (APCs). Parasite Immunol. 2003;25:199–209. 22. Rouas R, Lewalle P, Ouriaghli F, Nowak B, Duvillier H, Martiat P. Poly(I:C) used for human dendritic cell maturation preserves their ability to secondarily secrete bioactive IL-12. Int. Immunol. 2004;16:767-773. 23. Kima PE. The amastigote forms of Leishmania are experts at exploiting host cell processes to establish infection and persist. Int J Parasitol. 2007;37: 1087–1096. 24. Matte C, Olivier M. Leishmania- Induced Cellular Recruitment during the Early Inflammatory Response: Modulation of Proinflammatory Mediators. J Infect Dis. 2002;185:673–681. 25. Dasgupta B, Roychoudhury K, Ganguly S, Akbar M, Das P, Roy S. Infection of Human Mononuclear Phagocytes and Macrophage-Like THP1 Cells with Leishmania donovani Results in Modulation of Expression of a Subset of Chemokines and a Chemokine Receptor. Scand J Immunol. 2002;57:366–374. 26. Badolato R, Sacks D, Savoia D, Musso T. Leishmania major: Infection of Human Monocytes Induces Expression of IL-8 and MCAF. Exp Parasitol. 1996;82:21–26.

Format

application/pdf

Type of digital resource

info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
info:eu-repo/article/published
info:eu-repo/article/published
info:eu-repo/article/published

Células Dendríticas (CDs) diferenciadas a partir de Monocitos humanos como herramienta para el estudio de agentes antileishmaniales