Evaluación del crecimiento de cuatro especies del género Bacillus sp., primer paso para entender su efecto biocontrolador sobre Fusarium sp.

Castañeda Alvarez, Estefania; Sánchez, Ligia Consuelo

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Castañeda Alvarez, Estefania

Sánchez, Ligia Consuelo

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Universidad Colegio Mayor de Cundinamarca

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Abstract

Evaluar las condiciones de crecimiento de cuatro especies de Bacillus sp. nativas a escala de 10ml en Medio Mínimo de Sales (MMS) como primer paso para entender su acción biocontroladora contra Fusarium sp. Métodos. El procedimiento para evaluar el crecimiento de los aislamientos UCMC-TB1, UCMC-TB2, UCMC-TB3 y UCMC-TB4 se realizó utilizando espectrofotometría y recuento directo en placa y pruebas de antagonismo dual en placa para evaluar el efecto controlador contra Fusarium sp. Resultados. Se confirmó la identificación por pruebas bioquímicas de los cuatro aislamientos: Bacillus licheniformis, Bacillus subtilis, Bacillus pumilus y Bacillus cereus; todas las cepas presentaron antagonismo in vitro. El Bacillus subtilis fue la especie que demostró mayor capacidad antagónica (79,73%PICR) y las características más destacadas de esta cepa fueron su velocidad de crecimiento. El género Bacillus es uno de los más reportados para usar en el control biológico de hongos como Fusarium sp. el cual ataca un gran número de cultivos de interés económico para el sector agrícola en Colombia.

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