• español
    • English
    • français
    • português
A+A-
  • English 
    • español
    • English
    • français
    • português
    • Usage guides
      • Guidelines for the advisor work direcor
      • Guidelines for the student who loads degree work
      • APA 7 Edition Standards
      • Tips APA 7 Edition Standards
    • Users
    View Item 
    •   National Open and Distance University UNAD
    • Producción Científica
    • Sello Editorial UNAD
    • Revista Nova
    • View Item
    •   National Open and Distance University UNAD
    • Producción Científica
    • Sello Editorial UNAD
    • Revista Nova
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Lymphotherapy induce an increase of blocking factors and correct infertility problems

    Lymphotherapy induce an increase of blocking factors and correct infertility problems

    Lymphotherapy induce an increase of blocking factors and correct infertility problems

    Thumbnail
    QRCode
    Share
    Author
    Manrique, Edwin
    Rincón, Verónica
    Ossa, Humberto
    Publisher
    Universidad Colegio Mayor de Cundinamarca

    Citación

           
    TY - GEN T1 - Lymphotherapy induce an increase of blocking factors and correct infertility problems T1 - Lymphotherapy induce an increase of blocking factors and correct infertility problems AU - Manrique, Edwin AU - Rincón, Verónica AU - Ossa, Humberto UR - https://repository.unad.edu.co/handle/10596/29970 PB - Universidad Colegio Mayor de Cundinamarca AB - ER - @misc{10596_29970, author = {Manrique Edwin and Rincón Verónica and Ossa Humberto}, title = {Lymphotherapy induce an increase of blocking factors and correct infertility problemsLymphotherapy induce an increase of blocking factors and correct infertility problems}, year = {}, abstract = {}, url = {https://repository.unad.edu.co/handle/10596/29970} }RT Generic T1 Lymphotherapy induce an increase of blocking factors and correct infertility problems T1 Lymphotherapy induce an increase of blocking factors and correct infertility problems A1 Manrique, Edwin A1 Rincón, Verónica A1 Ossa, Humberto LK https://repository.unad.edu.co/handle/10596/29970 PB Universidad Colegio Mayor de Cundinamarca AB OL Spanish (121)
    Bibliographic managers
    Refworks
    Zotero / EndNote / Mendeley
    BibTeX
    CiteULike
    Metadata
    Show full item record
    Abstract
    This study aimed to confirm the presence of Blocking Factors (BFs) in Mixed Lymphocyte Culture (MLC) from female normal reproducer and sub-fertile rabbit inoculated with two injection of the allogenic lymphotherapy (LIT) to analyze its effect on rate fertility and pregnancy success. The BFs measuring was done intervening MLC with MTT-Formazan non-radioactive technique. It was demonstrated BFs presence in MLC in female rabbit groups.. In sub-fertile female reproducers treated with allogenic lymphotherapy a significant increase in the level of FBs after every LIT was observed, as well as a rate fertility increase.. Furthermore, it was established that BFs act on cell proliferation inhibiting the MLC of other species, clearly indicating that the inhibit effect of the BFs is inter-specific and no intraspecific as had sustain until now.
     
    This study aimed to confirm the presence of Blocking Factors (BFs) in Mixed Lymphocyte Culture (MLC) from female normal reproducer and sub-fertile rabbit inoculated with two injection of the allogenic lymphotherapy (LIT) to analyze its effect on rate fertility and pregnancy success. The BFs measuring was done intervening MLC with MTT-Formazan non-radioactive technique.It was demonstrated BFs presence in MLC in female rabbit groups.. In sub-fertile female reproducers treated with allogenic lymphotherapy a significant increase in the level of FBs after every LIT was observed, as well as a rate fertility increase.. Furthermore, it was established that BFs act on cell proliferation inhibiting the MLC of other species, clearly indicating that the inhibit effect of the BFs is inter-specific and no intraspecific as had sustain until now.
     
     
    College
    http://hemeroteca.unad.edu.co/index.php/nova/article/view/1020/1017
    /*ref*/Beer A, Billingham R.E. The embryo as a transplant. In: A Scientific American. 1974; 230: 36 - 46.
    /*ref*/Maldonado J. Efecto de la aloinmunoterapia en la reproducción animal. Tesis de grado como requisito parcial para optar al título de Magíster en Inmunología. Universidad de Antioquía. Facultad de Medicina. 1993
    /*ref*/Ossa J, Sánchez F, Cadavid A. Inmunología de la Reproducción. Primera Edición. Imprenta Universidad de Antioquía. Colombia. 1993. 206 p.
    /*ref*/Ossa H, Benítez A, Cárdenas S, Moreno C.H. Cuantificación de factores bloqueadores del cultivo mixto de linfocitos en mujeres abortadoras habituales tratadas con Linfoterapia. In: Medicina Reproductive. 2000; 3(1): 33 - 36
    /*ref*/Gurka G., Rocklin R.E. Reproductive Immunology. In: JAMA.1987;258 (20): 2983 – 7.
    /*ref*/Lancet. Maternal blocking antibodies, the fetal allograft and recurrent abortion. The Lancet. 1983.
    /*ref*/Mossmann T. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and citotoxicity assays. In: Journal of Immunological Methods. 1983;65: 55 - 63.
    /*ref*/PROMEGA. 1994. CELL TITER 96 TM Aqueous Non – Radioactive cell proliferation Assay. Technical Bulletin No 169.
    /*ref*/PROMEGA. 1995. CELL TITER 96 TM Non – Radioactive cell proliferation Assay. Technical Bulletin No 112.
    /*ref*/Daniel W. Bioestadística. Base para el análisis de ciencias de la salud. Editorial Uteha. Noriega editores. México. 1997; 878p.
    /*ref*/Reyes C. 1995. Bioestadística Aplicada. Agronomía, Biología, Química. Editorial Trillas. México. 216p.
    /*ref*/Mowbray J, Gibbings C; Liddell H; Reginald P; Underwood J; Beard R. Controlled trial of treatment of recurrent spontaneous abortion by immunisation with paternal cells. The Lancet. 1985; 8435: 941- 943.
    /*ref*/Ober C, Karrison T, Odem R; Barnes R, Branch W; Stephenson M, Baron B, Ann M, Scott J, Schreiber J. Mononuclear cell immunisation in prevention of recurrent miscarriages: a randomised trial. The Lancet. 1999; 354: 366 - 369.
    /*ref*/Adams C. E. Studies on prenatal mortality in the Rabbit, Oryctolagus cuniculus: The amount and distribution of loss before and after implantation. In: Journal. Endocrin. 1960;19: 325 -344.
    /*ref*/Adams C. E. Studies on prenatal mortality in the Rabbit, Oryctolagus cuniculus: The effect of transferring varying numbers of eggs. In: Journal of Endocrin. 1962; 24: 471 - 490.
    /*ref*/Adams C. E. Maintenance of pregnancy relative to the presence of few embryons in the rabbit. In: Journal of Endocrin. 1970; 48: 243 - 249.
    /*ref*/Benitez A., Cardenas. Determinación de factores bloqueadores mediante el cultivo mixto de linfocitos (CML), técnica MTT. Tesis de pregrado. Universidad Distrital Francisco José de Caldas. 2000.
    /*ref*/Scott Jr, Park M, Edwin S, Brach D. Interpretation of blocking activity in maternal serum depends on the equation used for calculation of mixed lymphocyte culture results. In: Clinical Experimental Immunology.1990;82: 363 - 368.
    Format
    application/pdf
    Type of digital resource
    info:eu-repo/semantics/article
    info:eu-repo/semantics/publishedVersion
    info:eu-repo/article/published
    info:eu-repo/article/published
    info:eu-repo/article/published
    URI
    https://repository.unad.edu.co/handle/10596/29970
    URL source
    http://hemeroteca.unad.edu.co/index.php/nova/article/view/1020
    http://dx.doi.org/10.22490/24629448.1020
    Collections
    • Revista Nova [1177]
    Usage guidesNormativityGuidelines for the advisor work direcorGuidelines for the student who loads degree workAPA 7 Edition StandardsTips APA 7 Edition Standards

    Browse

    All of DSpaceCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

    My Account

    LoginRegister
    GTM statisticsGTM statistics
    Indexed by:
    logo_Open Archives Initiative
    logo_Biblioteca Digital Ecuatoriana
    logo_OpenDOAR
    logo_Open ROAR
    logo_Google Scholar
    logo_Lyrasis
    logo_WorldCat
    logo_FAO
    logo_AGRIS
    logo_Alianza de Servicios de Información Agropecuaria
    logo_Siembra
    logo_Fedesarrollo
    logo_Colombia Digital
    logo_Hemeroteca UNAD
    logo_RED DE REPOSITORIOS LATINOAMERICANOS
    logo_OAIster
    logo_La Referencia
    logo_Open AIRE
    logo_Core
    logo_Base
    logo_CLACSO
    logo_OpenAlex
    logos isopreadGreat Work to PlaceIcontec - Great Work to Place

    Línea anticorrupción: 3232641617 ext. 1544

    En Bogotá D.C. (Colombia) Teléfono: 323 264 1617 - Línea gratuita nacional: 323 264 1617

    Institución de Educación Superior sujeta a inspección y vigilancia por el Ministerio de Educación Nacional

    Universidad Nacional Abierta y a Distancia UNAD de Colombia - © Copyright UNAD 2024

    Síguenos en: