Caracterización Molecular de Cepas Colombianas de . a Través del Rflp-is200
Molecular Characterization of Colombian Stumps of . Through the Rflp-is200
Molecular Characterization of Colombian Stumps of . Through the Rflp-is200
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Las técnicas moleculares empleadas para el análisis de ADN cromosomal como el RFLP IS200 han demostrado ser eficientes para resolver relaciones filogenéticas y epidemiológicas entre las cepas de Salmonella spp., aisladas de aves y humanos. El presente estudio tuvo como objetivo determinar si la IS200 estaba presente en las cepas de Salmonella aisladas de aves de corral y de humanos para establecer una posible cadena de transmisión entre ambos. El análisis filogenético de las cepas de Salmonella mostró 13 perfiles de IS200 que exhibieron de 1 a 11 copias de la secuencia de inserción, localizadas en un rango entre 13 y 1.71kb. El trabajo permitió concluir que IS200 es un marcador molecular eficiente, sensible y específico, útil para realizar estudios epidemiológicos ya que discriminó entre cepas de diferentes orígenes y comprobó que existe una relación clonal entre las cepas aviares y humanas. Los resultados obtenidos muestran la necesidad de implementar medidas sanitarias que conlleven a que en la producción se minimicen las condiciones que favorezcan la propagación de Salmonella entre las aves. Molecular techniques, used for the genomic DNA analysis, as IS200 RFLP has shown to be efficient to solve phylogenetic and epidemiologic relationship between Salmonella spp., strains, isolated from poultry and human. Present study had the aim to determine whether the IS200 sequence was present in the Salmonella spp., strains isolated from both origins, to establish a possible transmition chain between both. Phylogenetic analysis of Salmonella strains showed 13 IS200 profiles that exhibit between 1 and 11 copies of the insertion sequence, located in a range size between 13 and 1.7kb. This work allowed to conclude that IS200 is an efficient, sensible and specific molecular marker, useful to epidemiological studies because it capacity of discriminating between different origin strains and showed the existence of a relationship between this strains. The results obtained in this work showed the necessity of implementing sanitary rules to minimize the propagation of Salmonella spp., in the poultry production.
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http://hemeroteca.unad.edu.co/index.php/nova/article/view/17http://dx.doi.org/10.22490/24629448.17
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