Isolation of potential oil degrading bacteria in coastal habitats in Cartagena Bay, Colombia

Cabrera Ospina, Melody; Echeverri Jaramillo, Gustavo Eugenio; Manjarrez Paba, Ganiveth

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Cabrera Ospina, Melody

Echeverri Jaramillo, Gustavo Eugenio

Manjarrez Paba, Ganiveth

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Universidad Colegio Mayor de Cundinamarca

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Abstract

En esta investigación se aislaron bacterias de 4 hábitats en el ecosistema marino aledaño a una industria petroquímica en la Bahía de Cartagena. En el proceso, las muestras se sometieron a pre-enriquecimiento por una semana, a enriquecimiento por tres semanas y a un proceso de selección de cepas competitivas, donde se evidenciaron cambios marcados en las propiedades del crudo de petróleo, como en turbidez y agregados blancos por crecimiento bacteriano. Se aislaron diferentes morfotipos que al caracterizar bioquímicamente fueron identificados como Pseudomonas aeruginosa en todas las muestras, corroborando su gran capacidad de adaptación en ambientes contaminados de este tipo. Estos resultados permitirán la realización de pruebas de biodegradación con esta bacteria y desarrollar ensayos a nivel microcosmos para su uso potencial en procesos de biorremediación de aguas marinas contaminadas con petróleo.

In this investigation, bacteria were isolated from 4 habitats in the marine ecosystem adjacent to a petrochemical industry in the Bay of Cartagena (biofilms, neuston, surface water and sediments). In the process, the samples were subjected to a pre-enrichment of a week, to an enrichment of three weeks and a selection of competitive strains, where there were marked changes in the properties of crude oil, such as turbidity and white aggregates for bacterial growth. Different morph types were isolated to characterize biochemically as Pseudomonas aeruginosa was identified in all samples, confirming his great ability to adapt in such polluted environments. This will allow for biodegradation tests with this bacterium, so you can make at microcosm test for potential use in bioremediation of oil-contaminated marine waters.

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Aislamiento de bacterias potencialmente degradadoras de petróleo en hábitats de ecosistemas costeros en la Bahía de Cartagena, Colombia