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    Perspectivas en investigación: : células HEp-2 infectadas con EB’s de Chlamydia trachomatis serovar 2 (VR-902B)

    Chlamydia trachomatis: : HEp-2 Cells Infected with EB’s from Chlamydia trachomatis serovar 2 (VR-902B): Research Perspectives

    Chlamydia trachomatis: : HEp-2 Cells Infected with EB’s from Chlamydia trachomatis serovar 2 (VR-902B): Research Perspectives

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    Author
    Castellanos Hernández, Natalia
    Castañeda Franco, Yessica Marcela
    Caro Burgos, Paola Andrea
    Sánchez Mora, Ruth Mélida
    Publisher
    Universidad Colegio Mayor de Cundinamarca

    Citación

           
    TY - GEN T1 - Chlamydia trachomatis: : HEp-2 Cells Infected with EB’s from Chlamydia trachomatis serovar 2 (VR-902B): Research Perspectives T1 - Perspectivas en investigación: : células HEp-2 infectadas con EB’s de Chlamydia trachomatis serovar 2 (VR-902B) AU - Castellanos Hernández, Natalia AU - Castañeda Franco, Yessica Marcela AU - Caro Burgos, Paola Andrea AU - Sánchez Mora, Ruth Mélida UR - https://repository.unad.edu.co/handle/10596/37078 PB - Universidad Colegio Mayor de Cundinamarca AB - Chlamydia trachomatis (C. Trachomatis) is a Gram negative unmoving bacterium, characterized by being an obligate intracellular microorganism and having a reproductive cycle in which a metabolically inactive extracellular infectious form (elementary body - EB's) can be distinguished from an intracellular active and non-infectious form (reticulated body - RB's). C trachomatis is characterized by causing infection in humans, is related to sexually transmitted diseases and eye infections, so it can lead to sequelae of interest if timely treatment is not given. The objective of this study was to optimize the infection model of C. trachomatis in HEp-2 cells with elementary bodies (EB’s) of C. trachomatis serovar L2. Initially, the conditions for the adequate growth of HEp-2 cells were established in time and with a confluence of 90%, to continue with the optimization of an infection protocol. The infection was confirmed from the staining with Giemsa allowing to evaluate morphological characteristics of both uninfected and infected HEp-2 cells and also of the elementary bodies of C. trachomatis. Finally, the infection was corroborated with the direct immunofluorescence technique, that detects the C. trachomatis MOMP membrane protein. After the tests were performed, the presence of elementary bodies nearby and within the cellular cytoplasm was evidenced, as well as vacuolated cells and cellular damage caused by the infection. ER - @misc{10596_37078, author = {Castellanos Hernández Natalia and Castañeda Franco Yessica Marcela and Caro Burgos Paola Andrea and Sánchez Mora Ruth Mélida}, title = {Chlamydia trachomatis: : HEp-2 Cells Infected with EB’s from Chlamydia trachomatis serovar 2 (VR-902B): Research PerspectivesPerspectivas en investigación: : células HEp-2 infectadas con EB’s de Chlamydia trachomatis serovar 2 (VR-902B)}, year = {}, abstract = {Chlamydia trachomatis (C. Trachomatis) is a Gram negative unmoving bacterium, characterized by being an obligate intracellular microorganism and having a reproductive cycle in which a metabolically inactive extracellular infectious form (elementary body - EB's) can be distinguished from an intracellular active and non-infectious form (reticulated body - RB's). C trachomatis is characterized by causing infection in humans, is related to sexually transmitted diseases and eye infections, so it can lead to sequelae of interest if timely treatment is not given. The objective of this study was to optimize the infection model of C. trachomatis in HEp-2 cells with elementary bodies (EB’s) of C. trachomatis serovar L2. Initially, the conditions for the adequate growth of HEp-2 cells were established in time and with a confluence of 90%, to continue with the optimization of an infection protocol. The infection was confirmed from the staining with Giemsa allowing to evaluate morphological characteristics of both uninfected and infected HEp-2 cells and also of the elementary bodies of C. trachomatis. Finally, the infection was corroborated with the direct immunofluorescence technique, that detects the C. trachomatis MOMP membrane protein. After the tests were performed, the presence of elementary bodies nearby and within the cellular cytoplasm was evidenced, as well as vacuolated cells and cellular damage caused by the infection.}, url = {https://repository.unad.edu.co/handle/10596/37078} }RT Generic T1 Chlamydia trachomatis: : HEp-2 Cells Infected with EB’s from Chlamydia trachomatis serovar 2 (VR-902B): Research Perspectives T1 Perspectivas en investigación: : células HEp-2 infectadas con EB’s de Chlamydia trachomatis serovar 2 (VR-902B) A1 Castellanos Hernández, Natalia A1 Castañeda Franco, Yessica Marcela A1 Caro Burgos, Paola Andrea A1 Sánchez Mora, Ruth Mélida LK https://repository.unad.edu.co/handle/10596/37078 PB Universidad Colegio Mayor de Cundinamarca AB Chlamydia trachomatis (C. Trachomatis) is a Gram negative unmoving bacterium, characterized by being an obligate intracellular microorganism and having a reproductive cycle in which a metabolically inactive extracellular infectious form (elementary body - EB's) can be distinguished from an intracellular active and non-infectious form (reticulated body - RB's). C trachomatis is characterized by causing infection in humans, is related to sexually transmitted diseases and eye infections, so it can lead to sequelae of interest if timely treatment is not given. The objective of this study was to optimize the infection model of C. trachomatis in HEp-2 cells with elementary bodies (EB’s) of C. trachomatis serovar L2. Initially, the conditions for the adequate growth of HEp-2 cells were established in time and with a confluence of 90%, to continue with the optimization of an infection protocol. The infection was confirmed from the staining with Giemsa allowing to evaluate morphological characteristics of both uninfected and infected HEp-2 cells and also of the elementary bodies of C. trachomatis. Finally, the infection was corroborated with the direct immunofluorescence technique, that detects the C. trachomatis MOMP membrane protein. After the tests were performed, the presence of elementary bodies nearby and within the cellular cytoplasm was evidenced, as well as vacuolated cells and cellular damage caused by the infection. OL Spanish (121)
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    Abstract
    Chlamydia trachomatis (C. trachomatis) es una bacteria Gram negativa inmóvil, caracterizada por ser un microorganismo intracelular obligado y por poseer un ciclo reproductivo en el que puede distinguirse una forma infecciosa extracelular metabólicamente inerte (cuerpo elemental - EB’s), y una forma no infecciosa intracelular y activa (cuerpo reticulado - RB’s). C trachomatis se caracteriza por causar infección en humanos, está relacionada con enfermedades de transmisión sexual e infecciones oculares; por lo que puede conllevar a secuelas de interés, si no se da un tratamiento oportuno. El objetivo de este estudio fue optimizar el modelo de infección de C. trachomatis en células HEp-2 con cuerpos elementales (EB’s) de C. trachomatis serovar L2. Inicialmente, se establecieron las condiciones para el crecimiento adecuado de las células HEp-2 en tiempo y con una confluencia del 90%, para continuar con la optimización de un protocolo de infección. La infección fue confirmada a partir de la coloración con Giemsa permitiendo evaluar características morfológicas tanto de las células HEp-2 sin infectar e infectadas, y así mismo, de los cuerpos elementales de C. trachomatis. Finalmente, se corroboró la infección con la técnica de inmunofluorescencia directa que detecta la proteína de membrana MOMP de C. trachomatis. Tras los ensayos realizados se evidenció la presencia de cuerpos elementales próximos y dentro del citoplasma celular, así como células vacuoladas y daño celular causado por la infección.
     
    Chlamydia trachomatis (C. Trachomatis) is a Gram negative unmoving bacterium, characterized by being an obligate intracellular microorganism and having a reproductive cycle in which a metabolically inactive extracellular infectious form (elementary body - EB's) can be distinguished from an intracellular active and non-infectious form (reticulated body - RB's). C trachomatis is characterized by causing infection in humans, is related to sexually transmitted diseases and eye infections, so it can lead to sequelae of interest if timely treatment is not given. The objective of this study was to optimize the infection model of C. trachomatis in HEp-2 cells with elementary bodies (EB’s) of C. trachomatis serovar L2. Initially, the conditions for the adequate growth of HEp-2 cells were established in time and with a confluence of 90%, to continue with the optimization of an infection protocol. The infection was confirmed from the staining with Giemsa allowing to evaluate morphological characteristics of both uninfected and infected HEp-2 cells and also of the elementary bodies of C. trachomatis. Finally, the infection was corroborated with the direct immunofluorescence technique, that detects the C. trachomatis MOMP membrane protein. After the tests were performed, the presence of elementary bodies nearby and within the cellular cytoplasm was evidenced, as well as vacuolated cells and cellular damage caused by the infection.
     
     
    College
    https://hemeroteca.unad.edu.co/index.php/nova/article/view/3696/3762
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    application/pdf
    Type of digital resource
    info:eu-repo/semantics/article
    info:eu-repo/semantics/publishedVersion
    article
    article
    article
    URI
    https://repository.unad.edu.co/handle/10596/37078
    URL source
    https://hemeroteca.unad.edu.co/index.php/nova/article/view/3696
    10.22490/24629448.3696
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