Se diagnosticó en el programa de Bacteriología y Laboratorio Clínico al riesgo biológico como el de mayor incidencia en las prácticas académicas. Este diagnóstico fue sustentado por los análisis microbiológicos. Se procedió a aplicar la medida correctiva por acciones de prevención, conducentes al mejoramiento de la calidad de vida y desarrollo de la responsabilidad frente al cuidado del medio ambiente. El programa educativo que se aplicó se denominó Estrategias para una Nueva Cultura en Bioseguridad . Luego de ser aplicado a los diferentes estamentos universitarios se procedió a monitorear nuevamente la contaminación de los laboratorios, controlando la experiencia mediante la evaluación de las mediciones propuestas de eficacia, cobertura, eficiencia y efectividad. Se concluyó que la educación continuada y permanente desarrollada mediante un programa flexible, disminuyó significativamente la presencia de patógenos en los laboratorios.Once it was known the assessment about the problems related to the biological contamination that in the panorama of risks at the Health Sciences School highest incidence was found, the biological risk directly involving the performance of daily laboratory tasks. This finding was supported by the microbiological analysis and the prevention measures were implemented through an educational program, in order to reach a change towards a new culture of risks prevention, and to take care of oneself, to find a better quality of and to be able to develop the responsibility to take care of the environment. The educational program used was named Strategies for a New Biosecurity Culture and it was developed since the knowledge about the environment, the community and the agent. This educational program was implemented to teachers, to managing and to people who work in general services, then the evaluation was followed by an assessment of contamination levels in the laboratories, and this experience was evaluated by the indicator proposed, related to efficiency, efficacy and effectively.The results of the study was to know that a continuous and permanent educational program teaching since a flexible program development with special strategies that integrate about to be able to form an opinion, to be in the own context and to be innovating, and that if this knowledge is developed since the inducement, the present time, the new training and the evaluation so it was possible to obtain a significance decrease in the appearance of pathological microorganisms in labs.