Mycoses for Pythium insidiosum. First case with definitive diagnosis in Colombia

Acero-Mondragón MDV. Esp. MSc, Edward Javier; DaniBotero Rosas, MD. PhD, Daniel Alfonso; Maldonado Arango MD MSc, María Inés

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Acero-Mondragón MDV. Esp. MSc, Edward Javier

DaniBotero Rosas, MD. PhD, Daniel Alfonso

Maldonado Arango MD MSc, María Inés

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Universidad Colegio Mayor de Cundinamarca

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Abstract

Pythium insidiosum es un hongo distribuido globalmente causante de una micosis emergente llamada Pitiosis en personas y animales. El diagnóstico definitivo involucra correlación clínico epidemiológica, clínico patológica, histopatológica y molecular. En Colombia no existen antecedentes diagnósticos de Pitiosis con estas correlaciones. Pitiosis fue demostrada dos equinos de zona rural en Puerto Boyacá, Departamento de Boyacá-Colombia. Los equinos presentaron en extremidades lesiones únicas, pruriginosas, ulceradas, granulomatosas, alternada de masas sólidas con secreción serosanguinolenta. De las biopsias los hallazgos anatomopatológicos demostraron masas sólidas de aspecto coralino, las cuales se procesaron histopatológicamente. Los resultados correlacionados de biogeografía, las lesiones halladas en los equinos y en ellas los patrones histopatológicos de Splendore-Hoeplii, con hallazgos de hifas raramente septadas con diámetro de 2,5 a 6,2 micras en ramificación angulada, junto a la secuenciación del DNA extraído y comparado con el depositado para P.insidiosum en el GenBank permitió demostrar en todas las muestras una homología del 100% conformándose por primera vez el diagnóstico definitivo de Pitiosis en Colombia.

Pythium insidiosum is a globally distributed fungus that causes a fungal infection called Pythio-sis is considered an emerging disease in humans and animals. A definitive diagnosis involves clinical, epidemiological, histopathological and molecular correlations. There are no reports of Pythiosis diagnosis involving all these correlations in Colombia. In this study were confirmed Phytiosis in two horses in rural area of Puerto Boyaca (Boyaca, Colombia) presenting single,itching, ulcerated, granulomatous lesions on their limbs with serosanguineous suppuration. Biopsies were analyzed pathologically and histopathologically. Polymerase Chain Reaction (PCR) was carried out from DNA extracted of paraffin blocks. This study report the first definitive diagnosis of phytiosis in Colombia based on the correlation between bio-geography area, the pathological and histopathological findings which included patterns like Splendore-Hoeplii and hyphae with rare septae (2.5 to 6.2 microns diameters of branching angled) along with sequenced DNA that showed 100% homology with the Pythium insidiosum sequence at the GenBank.

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http://hemeroteca.unad.edu.co/index.php/nova/article/view/1021/1018
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Micosis por Pythium Insidiosum primer caso con diagnóstico definitivo en Colombia