a multiresistencia a los antibióticos por bacterias Gram positivas, especialmente el Staphylococcus epidermidis, constituye un problema creciente de salud pública en las unidades neonatales. Uno de los mecanismos de resistencia bacteriana recientemente descrito es la presencia de integrones y cassettes genéticos. Existen más de 9 clases de integrones, siendo el de clase 1 el que con mayor frecuencia se encuentra implicado en cepas causantes de infección hospitalaria.En este estudio, se determinó la presencia de integrones clase 1 en Staphylococcus epidermidis, provenientes de hemocultivos y puntas de catéter, que causaron infección nosocomial en las unidades neonatales del Instituto Materno Infantil de Bogotá, Colombia. De las 46 cepas estudiadas, se detectó la presencia del gen intI 1 en 21 de los aislamientos (45.7%). Este hallazgo, pionero en cepas bacterianas aisladas en neonatos, sugiere la necesidad de buscar cassettes genéticos que confieren resistencia a los diferentes antimicrobianos, para definir esquemas de tratamiento de infecciones nosocomiales causadas por estos microorganismos.Multiresistance to antimicrobial against Gram-positive bacteria, especially Staphylococcus epidermidis is a progressive problem in newborn-intensive care units. One of the recently described mechanisms of bacterial ressistance is the presence of integrons and gene cassettes. There are more than 9 types of integrons, out of which class 1 integron is the most frequently associated with strains causing hospital infections.In this study, presence of class 1 integrons in Staphylococcus epidermis was determined as coming from hemocultures and catheter points which caused nasocomial infection in newborn intensive care units of Instituto Materno Infantil in Bogotá, Colombia. From the 46 studied strains, intl1 gene was detected in 21 of them (45.7%). This finding, pioneer in bacterial strains isolated in newborns, suggests the necessity to look for gene cassette which give resistance to the different anti microbial entities to define nosocomial infection treatment schemes caused by these microorganisms.This is the first time mechanism of resistance to aminoglycoside and other antimicrobials in Colombian Gram positive strains is studied. We suggest that it is important to carry out assays with gene cassette for different antibiotics of nosocomial infection to provide a better treatment selection.